Crown Copyright (C) 2011 Published by Elsevier Ltd. fowleri from the environment may aid ecological studies and enable intervention to prevent PAM cases. The availability of a rapid, reliable and sensitive one-step nested PCR method for the direct detection of N. fowleri or other thermophilic Naegleria spp. Although culture of samples yielded numerous thermophilic free-living amoebae, none were N. ![]() Here, the assay detected N, fowleri in 18/109 river water samples associated with a nuclear power plant in South West France and 0/10 from a similar site in the UK. Here, a nested one-step PCR test, in conjunction with a direct DNA extraction from water or sediment material, was developed for the rapid and reliable detection of N. fowleri but requires several days incubation and subsequent biochemical or molecular tests to confirm identification. Culture of environmental material is the conventional method for the isolation of N. fowleri in the interests of preventive public health microbiology. It is, therefore, important to identify sites containing N. Infection typically results from bathing in contaminated water and is usually fatal. The organism is pathogenic to man causing fatal primary amoebic meningoencephalitis (PAM). Naegleria fowleri is a small free-living amoebo-flagellate found in natural and manmade thermal aquatic habitats worldwide.
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